孔德昭

食品中真菌毒素、微生物、过敏原等危害物质检测方法开发

申请人2012年9月就读于江南大学食品科学与工程学院，期间主要从事食品质量安全检测方向工作，主要研究内容包括电化学检测传感器快速检测技术的开发，食品中主要污染真菌毒素、致病菌、过敏原、营养物质等单克隆抗体制备及免疫学检测方法建立与研究，以第一作者在Nanoscale、Analytical Methods，Food and Agricultural Immunology等国际期刊发表SCI论文十余篇，授权国家专利十余项，获得江南大学优秀博士论文。2018年1月就职于江苏科技大学粮食学院，主要从事粮食中真菌毒素降解研究与粮油危害物质检测方法建立研究，研究内容得到江苏省基础研究计划（自然科学基金）、江苏省高等学校自然科学研究计划、江苏科技大学人才启动经费计划等项目的资助，于Food Chemistry等杂志发表论文，并申请国家专利。期间于国家粮食和物资储备局科学研究院挂职工作一年，参与多项市场食品快速检测产品验证评价工作以及参与多项十三五国家重点研发计划项目申报工作；入选2019年江苏省“双创计划”科技副总项目；获得江苏科技大学2019年新兴科技创新培育计划负责人。

团队名称

粮油食品安全检测团队

团队主要研究方向：

食品中真菌毒素、微生物、过敏原等危害物质检测方法开发

团队主要成果：

(1) Dezhao Kong ; Liqiang Liu; Shanshan Song; Steven Suryoprabowo; Aike Li; Hua Kuang; Libing Wang; Chuanlai Xu , A gold nanoparticle-based

semi-quantitative and quantitative ultrasensitive paper sensor for the detection of twenty mycotoxins, Nanoscale, 2016.2, 8(9): 5245~5253

(2) Dezhao Kong ; Xiaoling Wu; Yue Li; Liqiang Liu; Shanshan Song; Qiankun Zheng; Hua Kuang ; Chuanlai Xu , Ultrasensitive and eco-friendly immunoassays based monoclonal antibody for detection of deoxynivalenol in cereal and feed samples, Food Chemistry, 2018.7.11, 270: 130~137

(3) Dezhao Kong ; Liu, Liqiang; Song, Shanshan; Kuang, Hua; Xu, Chuanlai , Development of an immunochromatographic strip for the semi-quantitative and

quantitative detection of biotin in milk and milk products, Analytical Methods,2016.1, 8(7): 1595~1601

(4) Kong, Dezhao ; Liqiang Liu; Shanshan Song; Hua Kuang ; Chuanlai Xu , Development of sensitive, rapid, and effective immunoassays for the detection

of vitamin B-12 in fortified food and nutritional supplements, Food Analytical

Methods, 2017.1, 10(1): 10~18

(5) Yaqi Li, Li Sun, Dezhao Kong, Jianguo Sheng, Kun Wang, Chunwang Dong*. A Green, Simple, and Rapid Detection for Amaranth in Candy Samples Based on the Fluorescence Quenching of Nitrogen-Doped Graphene Quantum Dots. Food Analytical Methods, 12(7), 1658-1665.

(6) Yaqi Li, Li Sun, Jianrong Cai, and Kun Wang， Fluorescent “on-off-on” switching sensor based on CdTe quantum dots coupled with multiwalled carbon nanotubes@graphene oxide nanoribbons for simultaneous monitoring of dual foreign DNAs in transgenic soybean, Biosensors and Bioelectronics, 2017, 92, 26-32.

(7) Yaqi Li, Li Sun, Jianrong Cai, and Kun Wang, A homogeneous assay for highly sensitive detection of CaMV35S promoter in transgenic soybean by förster resonance energy transfer between nitrogen-doped graphene quantum dots and Ag nanoparticles, Analytica Chimica Acta, 2016, 948, 90-97.

(8) Yaqi Li, Li Sun, Jianrong Cai, Kun Wang, Photoelectrochemical CaMV35S biosensor for discriminating transgenic from non-transgenic soybean based on SiO2@CdTe quantum dots core-shell nanoparticles as signal indicators, Talanta, 2016, 161, 211–218.

(9) Hua Ye, Qianqian Lu, Nuo Duan, Zhouping Wang. GO-amplified fluorescence polarization assay for high-sensitivity detection of Aflatoxin B1 with low dosage aptamer probe. Analytical and Bioanalytical Chemistry, 2019, 411(5):1107-1115.

(10) Hua Ye, Nuo Duan, Huajie Gu, Haitao Wang, Zhouping Wang. Fluorometric determination of lipopolysaccharides via changes of the graphene oxide-enhanced fluorescence polarization caused by truncated aptamers. Microchimica Acta, 2019. DOI: 10.1007/s00604-019-3261-8.

(11) Zhong, J. J., Liao, N., Li, C., Wang, W., Wang, D., Pu, Y., Ye, X., Liu, D.* (2019). Acinetobacter as a potentially important producer of urocanic acid in chub mackerel, a histidine metabolite of emerging health concern. European Food Research and Technology, 245(4), 825–835.

(12) Zhong, J. J., Liao, N., Pu, Y., Ding, T., Ye, X., Liu, D.* (2018). HTLC: A viable approach to fast LC of biogenic amines in food with legacy equipment. Chromatographia, 81, 1097-1102.

(13) Zhong, J. J., Liao, N., He, M., Pu, Y., Liu, D.* (2018). Development of an analytical method for urocanic acid isomers in fish based on reactive extraction cleanup and chaotropic chromatography techniques. Journal of Chromatography A, 1548, 44-50.

(14) Tang Sheng*, Xia Dasha, Yao Yao, Chen Tianyu, Sun Jun, Yin Yijie, Shen Wei, Peng Yinxian, Dye adsorption by self-recoverable, adjustable amphiphilic graphene aerogel, Journal of Colloid and Interface Science, 554 (2019) 682-691

(15) Tang Sheng*, Li Yana, Zhu Anni, Yao Yao, Sun Jun, Zheng Fenfen, Lin Zixia, Shen Wei*, A triple-amplification strategy based on the formation of peroxidase-like two dimensional DNA/Fe3O4 networks initiated by hybridization chain reaction for highly sensitive detection of microRNA, Chemical Communications, 55 (2019) 8386-8389 Back Cover

(16) Tang Sheng, Qi Tong, Xia Dasha, Xu Mengchan, Xu Mengyuan, Zhu Anni, Shen Wei, Lee Hian Kee*, Smartphone nanocolorimetric determination of hydrogen sulfide in biosamples after silver-gold core-shell nanoprism-based headspace single drop microextraction, Analytical Chemistry, 91 (2019) 5888-5895 , DOI: 10.1021/acs.analchem.9b00255

(17) Tang Sheng, Sun Jun, Xia Dasha, Zang Bin, Gao Yuhua, Chen Chuanxiang*, Shen Wei, Lee Hian Kee*, In-syringe extraction using compressible and self-recoverable, amphiphilic graphene aerogel as sorbent for determination of phenols. Talanta 195(2019) 165-172.

(18) Tang Sheng, Zhang Hong, Lee Hian Kee. Advances in sample extraction. Analytical Chemistry 88 (2016) 228-249. Invited review

(19) Wei Shen, Zhiqiang Gao, Quantum dots and duplex-specific nuclease enabled ultrasensitive detection and serotyping of Dengue viruses in one step in a single tube, Biosens. Bioelectron. 65 (2015) 327-332.

(20) Wei Shen, Ye Tian, Tong Ran, Zhiqiang Gao, Genotyping and qu